DNA repair protocols /
DNA repair protocols /
edited by Lotte Bjergb�k.
- 3rd ed.
- New York : Humana Press/Springer, ©2012.
- xvii, 631 p. : ill. (some col.) ; 27 cm.
- Methods in molecular biology, 920 1064-3745 ; Springer protocols .
- Methods in molecular biology (Clifton, N.J.) ; 920. Springer protocols. .
Includes bibliographical references and index.
DNA repair : front and center and not going away! / Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome / Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening / Establishment of the DNA repair-defective mutants in DT40 cells / Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair / Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair / Detection of DNA damage by comet fluorescence in situ hybridization / Quantitative DNA damage and repair measurement with the yeast comet assay / Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR / In vitro DNA mismatch repair in human cells / Eukaryotic DNA mismatch repair in vitro / Immuno-slot blot assay for detection of UVR-mediated DNA damage / Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay / Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR / Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies / Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts / In vitro base excision repair using mammalian cell extracts / Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro / In vitro monitoring of base excision repair in Saccharomyces cerevisiae / Mitochondrial base excision repair assays / O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks / DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis / Quantitative amplification of single-stranded DNA / Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast / Monitoring DNA recombination initiated by HO endonuclease / Monitoring I-SceI-induced double-strand break repair in DT40 Cells / I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks / Studying repair of a single protein-bound nick in vivo using the Flp-nick system / Measurement of diversification in the immunoglobulin light chain gene of DT40 cells / Live cell microscopy of DNA damage response in Saccharomyces cerevisiae / Use of gene targeting to study recombination in mammalian cell DNA repair mutants / In vitro rejoining of double strand breaks in genomic DNA / An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides / Assays of bypass replication of genotoxic lesions in cell-free extracts / Quantitative measurement of translesion DNA synthesis in mammalian cells / Detection of PCNA modifications in Saccharomyces cerevisiae / In vitro PCNA modification assays / Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / CHK1 kinase activity assay / Histone H2AX phosphorylation : a marker for DNA damage / Vilhelm A. Bohr -- Dashnamoorthy Ravi and Alexander James Roy Bishop -- Lone Vedel Sch�ler, Tine H�rning M�ller, Steffen N�rgaard, Lotte Vestergaard, and Anders Olesn -- Masamichi Ishiai, Emi Uchida, and Minoru Takata -- Chun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu -- G�unter Speit and Andreas Rothfuss -- Wiebke Schl�ormann and Michael Glei -- Rui Oliveira and Bj�orn Johansson -- Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten -- Liya Gu, Charles M. Ensor, and Guo-Min Li -- Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang -- Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke -- Thomas R. Berton and David L. Mitchell -- Ahmad Besaratinia and Gerd P. Pfeifer -- Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Sch�arer -- Puck Knipscheer, Markus R�aschle, Orlando Sch�arer, and Johannes C. Walter -- Jason L. Parsons and Grigory L. Dianov -- Dolores C�ordoba-Ca�nero, Teresa Rold�an-Arjona, and Rafael R. Ariza -- Simone Moertl and Fred Ahne -- Ricardo Gredilla and Tinna Stevnsner -- April M. Luke and Jun Nakamura -- Helen E. Bryant -- Eva-Maria Holstein and David Lydall -- James M. Dewar and David Lydall -- Neal Sugawara and James E. Haber -- David du Plessis and Julian E. Sale -- Amanda Gunn and Jeremy M. Stark -- Ida Nielsen, Anni H. Andersen, and Lotte Bjergb�k -- Julian E. Sale -- Sonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby -- Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn -- George Iliakis, Emil Mladenov, and Nge Cheong -- Kamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters -- Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone -- Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh -- Adelina A. Davies and Helle D. Ulrich -- Joanne L. Parker and Helle D. Ulrich -- George Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang -- Hong Yan Wang and Ya Wang -- Arishya Sharma, Kamini Singh, and Alexandru Almasan.
9781617799976 (hbk. : alk. paper) 1617799971 (hbk. : alk. paper)
DNA repair--Laboratory manuals.
DNA Repair--Laboratory Manuals.
572.86459 / DNA
Includes bibliographical references and index.
DNA repair : front and center and not going away! / Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome / Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening / Establishment of the DNA repair-defective mutants in DT40 cells / Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair / Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair / Detection of DNA damage by comet fluorescence in situ hybridization / Quantitative DNA damage and repair measurement with the yeast comet assay / Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR / In vitro DNA mismatch repair in human cells / Eukaryotic DNA mismatch repair in vitro / Immuno-slot blot assay for detection of UVR-mediated DNA damage / Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay / Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR / Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies / Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts / In vitro base excision repair using mammalian cell extracts / Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro / In vitro monitoring of base excision repair in Saccharomyces cerevisiae / Mitochondrial base excision repair assays / O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks / DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis / Quantitative amplification of single-stranded DNA / Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast / Monitoring DNA recombination initiated by HO endonuclease / Monitoring I-SceI-induced double-strand break repair in DT40 Cells / I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks / Studying repair of a single protein-bound nick in vivo using the Flp-nick system / Measurement of diversification in the immunoglobulin light chain gene of DT40 cells / Live cell microscopy of DNA damage response in Saccharomyces cerevisiae / Use of gene targeting to study recombination in mammalian cell DNA repair mutants / In vitro rejoining of double strand breaks in genomic DNA / An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides / Assays of bypass replication of genotoxic lesions in cell-free extracts / Quantitative measurement of translesion DNA synthesis in mammalian cells / Detection of PCNA modifications in Saccharomyces cerevisiae / In vitro PCNA modification assays / Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / CHK1 kinase activity assay / Histone H2AX phosphorylation : a marker for DNA damage / Vilhelm A. Bohr -- Dashnamoorthy Ravi and Alexander James Roy Bishop -- Lone Vedel Sch�ler, Tine H�rning M�ller, Steffen N�rgaard, Lotte Vestergaard, and Anders Olesn -- Masamichi Ishiai, Emi Uchida, and Minoru Takata -- Chun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu -- G�unter Speit and Andreas Rothfuss -- Wiebke Schl�ormann and Michael Glei -- Rui Oliveira and Bj�orn Johansson -- Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten -- Liya Gu, Charles M. Ensor, and Guo-Min Li -- Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang -- Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke -- Thomas R. Berton and David L. Mitchell -- Ahmad Besaratinia and Gerd P. Pfeifer -- Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Sch�arer -- Puck Knipscheer, Markus R�aschle, Orlando Sch�arer, and Johannes C. Walter -- Jason L. Parsons and Grigory L. Dianov -- Dolores C�ordoba-Ca�nero, Teresa Rold�an-Arjona, and Rafael R. Ariza -- Simone Moertl and Fred Ahne -- Ricardo Gredilla and Tinna Stevnsner -- April M. Luke and Jun Nakamura -- Helen E. Bryant -- Eva-Maria Holstein and David Lydall -- James M. Dewar and David Lydall -- Neal Sugawara and James E. Haber -- David du Plessis and Julian E. Sale -- Amanda Gunn and Jeremy M. Stark -- Ida Nielsen, Anni H. Andersen, and Lotte Bjergb�k -- Julian E. Sale -- Sonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby -- Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn -- George Iliakis, Emil Mladenov, and Nge Cheong -- Kamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters -- Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone -- Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh -- Adelina A. Davies and Helle D. Ulrich -- Joanne L. Parker and Helle D. Ulrich -- George Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang -- Hong Yan Wang and Ya Wang -- Arishya Sharma, Kamini Singh, and Alexandru Almasan.
9781617799976 (hbk. : alk. paper) 1617799971 (hbk. : alk. paper)
DNA repair--Laboratory manuals.
DNA Repair--Laboratory Manuals.
572.86459 / DNA