MARC details
000 -LEADER |
fixed length control field |
07425nam a22006617a 4500 |
001 - CONTROL NUMBER |
control field |
0076334 |
003 - CONTROL NUMBER IDENTIFIER |
control field |
BD-SySUS |
005 - DATE AND TIME OF LATEST TRANSACTION |
control field |
20220613092743.0 |
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS |
fixed length control field |
m o d |
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION |
fixed length control field |
cr cnu---unuuu |
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION |
fixed length control field |
140623s2014 onc o 000 0 eng d |
020 ## - INTERNATIONAL STANDARD BOOK NUMBER |
International Standard Book Number |
9780124095366 |
Qualifying information |
(electronic bk.) |
|
International Standard Book Number |
0124095364 |
Qualifying information |
(electronic bk.) |
|
International Standard Book Number |
9780124104907 |
Qualifying information |
(electronic bk.) |
|
International Standard Book Number |
0124104908 |
Qualifying information |
(electronic bk.) |
|
Canceled/invalid ISBN |
9780124095366 |
|
Canceled/invalid ISBN |
0124095364 |
035 ## - SYSTEM CONTROL NUMBER |
System control number |
(OCoLC)881571700 |
Canceled/invalid control number |
(OCoLC)961202138 |
-- |
(OCoLC)1076563831 |
-- |
(OCoLC)1105197705 |
-- |
(OCoLC)1105574825 |
040 ## - CATALOGING SOURCE |
Original cataloging agency |
EBLCP |
Language of cataloging |
eng |
Description conventions |
pn |
Transcribing agency |
EBLCP |
Modifying agency |
OPELS |
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N$T |
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UIU |
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OCLCF |
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OCLCQ |
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DEBSZ |
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DEBBG |
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OCLCQ |
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ITD |
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YDX |
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MERER |
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OCLCQ |
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ESU |
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OCLCQ |
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OCLCO |
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OCL |
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OCLCO |
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MERUC |
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OCLCQ |
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U3W |
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D6H |
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CHVBK |
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UUM |
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OTZ |
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OCLCQ |
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LQU |
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OCLCQ |
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OCLCO |
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OCLCA |
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S2H |
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OCLCO |
-- |
BD-SySUS |
049 ## - LOCAL HOLDINGS (OCLC) |
Holding library |
EQOA |
050 #4 - LIBRARY OF CONGRESS CALL NUMBER |
Classification number |
QH442.6 |
060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER |
Classification number |
QY 25 |
072 #7 - SUBJECT CATEGORY CODE |
Subject category code |
SCI |
Subject category code subdivision |
013060 |
Source |
bisacsh |
|
Subject category code |
TEC |
Subject category code subdivision |
009010 |
Source |
bisacsh |
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER |
Classification number |
660.65 |
Edition number |
23 |
Item number |
TRA |
092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC) |
Classification number |
WMS881571700 |
245 00 - TITLE STATEMENT |
Title |
Transgenic animal technology : |
Remainder of title |
a laboratory handbook / |
Statement of responsibility, etc. |
edited by Carl A. Pinkert. |
250 ## - EDITION STATEMENT |
Edition statement |
3rd ed. |
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT) |
Place of publication, distribution, etc. |
Burlington : |
Name of publisher, distributor, etc. |
Elsevier Science, |
Date of publication, distribution, etc. |
2014. |
300 ## - PHYSICAL DESCRIPTION |
Extent |
715 p. ; |
Other physical details |
ill.; |
Dimensions |
24 cm. |
336 ## - CONTENT TYPE |
Content type term |
text |
Content type code |
txt |
Source |
rdacontent |
337 ## - MEDIA TYPE |
Media type term |
computer |
Media type code |
c |
Source |
rdamedia |
338 ## - CARRIER TYPE |
Carrier type term |
online resource |
Carrier type code |
cr |
Source |
rdacarrier |
490 1# - SERIES STATEMENT |
Series statement |
Elsevier insights |
500 ## - GENERAL NOTE |
General note |
Section One Overview. Introduction to transgenic animals. Section Two Transgenic animal production focusing on the mouse model. Transgenic animal production using DNA microinjection. Factors affecting transgenic animal production. Gene targeting in embryonic stem cells I: History and methodology. Gene targeting in embryonic stem cells II. Conditional technologies. Retrovirus-mediated gene transfer. Nuclear transfer technologies. Section Three Production of transgenic laboratory and domestic animal species. Production of transgenic rats. Production of transgenic rabbits. Production of transgenic fish. Production of transgenic poultry. Production of transgenic swine by DNA Microinjection. Production of transgenic ruminants by DNA Microinjection. Production of transgenic non-human primates. Retrovirus-mediated gene transfer into oocytes. Section Four: Molecular biology, analyses and enabling technologies Vector design for transgene expression. Analysis of transgene integration. PCR optimization for detection of transgenic integration. Evaluation of transgene expression. Control of gene expression (from standard gain/loss of function to anti-sense to PNAs). Enabling technologies: Cryopreservation of gametes. Enabling technologies: Embryo culture considerations. Databases, Internet Resources and Genetic Nomenclature. |
505 0# - FORMATTED CONTENTS NOTE |
Formatted contents note |
Front Cover; Transgenic Animal Technology; Copyright Page; Contents; List of Contributors; Preface; One: Overview; 1 Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; Two: Transgenic Animal Production Focusing on the Mouse Model; 2 DNA Microinjection, Embryo Handling, and Germplasm Preservation; I. Introduction; II. General Methods; A. DNA Preparation and Purification; 1. DNA Construct/Fragment Structure; 2. Factors that Influence Transgenic Mouse Production; 3. Preparation of DNA for Microinjection. |
|
Formatted contents note |
A. Construction and Isolation of DNAb. DNA Purification; c. Purification and Quantification of DNA; B. Superovulation, Egg Culture, and Harvest; 1. Mouse Strains for Microinjection and Transgenic Models; a. Strains; b. Genetic Variability and Transgene Expression; 2. Superovulation of Mice; a. Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin; b. Follicle Stimulating Hormone and Luteinizing Hormone Delivered with Osmotic Pumps; 3. Production of Eggs from Superovulated Females; a. Colony; b. Influence of Litter Number; 4. In Vitro Culture of Superovulated Eggs; a. Equipment. |
|
Formatted contents note |
B. Atmosphere for Culturing Eggsc. Glass Pipettes for Manipulating Eggs; d. Media; e. Water Sources; f. Culture Dishes; 5. Harvesting Superovulated Eggs; a. Mating; b. Oviduct Dissection; c. Release of Eggs from the Oviduct; d. Egg Development and Microinjection Timing; e. Efficiency of Egg Culture; C. Microinjection Needles and Slides; 1. Types of Glass; 2. Preparation of Glass; 3. Pipette Pullers; 4. Injection Pipette Shapes; 5. Beveling Hollow Capillary Microinjection Needles; 6. Microforge Shaping of Pipettes; a. Manufacturers; b. Heating Filament; c. Holding Pipette; d. Injection Pipette. |
|
Formatted contents note |
7. Microinjection Slide for Eggsa. Slide Designs; b. Microinjection Media; c. Arrangement of DNA and Media Drops; D. Microinjection Equipment; 1. Microscopes; 2. Micromanipulators; a. Micropipette Holders; b. Micromanipulator Adjustments; c. Antivibration Tables; 3. Microinjection Systems; a. General; b. Oil-Driven Injection Assembly; 4. Oil-Driven Holding Pipettes; 5. Electronic Delivery Systems; E. Microinjection Procedure; 1. Preparation of Injection and Holding Pipettes (Micrometer Syringe System); a. Holding Pipette; b. Injection Pipette; c. Aligning the Injection Needle. |
|
Formatted contents note |
D. Loading DNA into the Injection Needle2. Preparing Eggs for Microinjection; 3. Steps for Efficient Manipulation of Eggs During Microinjection; a. General; b. Micromanipulators; c. Microinjection Needle; d. Controls and Egg Manipulation; 4. Visualizing and Injecting the Egg Pronuclei; a. Orientation; b. Egg Membrane Penetration with the Injection Needle; c. Filling the Pronuclei with DNA Solution; 5. Criteria for Microinjection to Yield Transgenic Mice; a. Treatment After Microinjection; b. Egg Lysis; c. Record Keeping; d. In Vitro Culture of Microinjected Eggs; F. Egg Transfer. |
520 ## - SUMMARY, ETC. |
Summary, etc. |
Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engine. |
588 ## - SOURCE OF DESCRIPTION NOTE |
Source of description note |
Description based on print version record. |
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM |
Topical term or geographic name as entry element |
Animal genetic engineering. |
9 (RLIN) |
38568 |
|
Topical term or geographic name as entry element |
Transgenic animals. |
9 (RLIN) |
38569 |
|
Topical term or geographic name as entry element |
Animal biotechnology. |
9 (RLIN) |
38570 |
|
Topical term or geographic name as entry element |
Animals, Genetically Modified. |
9 (RLIN) |
38571 |
|
Topical term or geographic name as entry element |
SCIENCE |
General subdivision |
Chemistry |
-- |
Industrial & Technical. |
Source of heading or term |
bisacsh |
9 (RLIN) |
38572 |
|
Topical term or geographic name as entry element |
TECHNOLOGY & ENGINEERING |
General subdivision |
Chemical & Biochemical. |
Source of heading or term |
bisacsh |
9 (RLIN) |
38573 |
|
Topical term or geographic name as entry element |
Animal biotechnology. |
Source of heading or term |
fast |
Authority record control number |
(OCoLC)fst00809103 |
9 (RLIN) |
38570 |
|
Topical term or geographic name as entry element |
Animal genetic engineering. |
Source of heading or term |
fast |
Authority record control number |
(OCoLC)fst00809192 |
9 (RLIN) |
38568 |
|
Topical term or geographic name as entry element |
Transgenic animals. |
Source of heading or term |
fast |
Authority record control number |
(OCoLC)fst01154666 |
9 (RLIN) |
38569 |
655 #2 - INDEX TERM--GENRE/FORM |
Genre/form data or focus term |
Laboratory Manual. |
9 (RLIN) |
38574 |
700 1# - ADDED ENTRY--PERSONAL NAME |
Personal name |
Pinkert, Carl A., |
Relator term |
editor. |
9 (RLIN) |
38575 |
776 08 - ADDITIONAL PHYSICAL FORM ENTRY |
Relationship information |
Erscheint auch als: |
Note |
Druck-Ausgabe |
Main entry heading |
Pinkert, Carl A. Transgenic Animal Technology . |
Title |
A Laboratory Handbook |
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE |
Uniform title |
Elsevier insights. |
9 (RLIN) |
31665 |
856 40 - ELECTRONIC LOCATION AND ACCESS |
Materials specified |
ScienceDirect All Books |
Uniform Resource Identifier |
<a href="https://www.sciencedirect.com/science/book/9780124104907">https://www.sciencedirect.com/science/book/9780124104907</a> |
942 ## - ADDED ENTRY ELEMENTS (KOHA) |
Source of classification or shelving scheme |
Dewey Decimal Classification |
Koha item type |
Books |