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Transgenic animal technology : (Record no. 76300)

MARC details
000 -LEADER
fixed length control field 07425nam a22006617a 4500
001 - CONTROL NUMBER
control field 0076334
003 - CONTROL NUMBER IDENTIFIER
control field BD-SySUS
005 - DATE AND TIME OF LATEST TRANSACTION
control field 20220613092743.0
006 - FIXED-LENGTH DATA ELEMENTS--ADDITIONAL MATERIAL CHARACTERISTICS
fixed length control field m o d
007 - PHYSICAL DESCRIPTION FIXED FIELD--GENERAL INFORMATION
fixed length control field cr cnu---unuuu
008 - FIXED-LENGTH DATA ELEMENTS--GENERAL INFORMATION
fixed length control field 140623s2014 onc o 000 0 eng d
020 ## - INTERNATIONAL STANDARD BOOK NUMBER
International Standard Book Number 9780124095366
Qualifying information (electronic bk.)
International Standard Book Number 0124095364
Qualifying information (electronic bk.)
International Standard Book Number 9780124104907
Qualifying information (electronic bk.)
International Standard Book Number 0124104908
Qualifying information (electronic bk.)
Canceled/invalid ISBN 9780124095366
Canceled/invalid ISBN 0124095364
035 ## - SYSTEM CONTROL NUMBER
System control number (OCoLC)881571700
Canceled/invalid control number (OCoLC)961202138
-- (OCoLC)1076563831
-- (OCoLC)1105197705
-- (OCoLC)1105574825
040 ## - CATALOGING SOURCE
Original cataloging agency EBLCP
Language of cataloging eng
Description conventions pn
Transcribing agency EBLCP
Modifying agency OPELS
-- N$T
-- UIU
-- OCLCF
-- OCLCQ
-- DEBSZ
-- DEBBG
-- OCLCQ
-- ITD
-- YDX
-- MERER
-- OCLCQ
-- ESU
-- OCLCQ
-- OCLCO
-- OCL
-- OCLCO
-- MERUC
-- OCLCQ
-- U3W
-- D6H
-- CHVBK
-- UUM
-- OTZ
-- OCLCQ
-- LQU
-- OCLCQ
-- OCLCO
-- OCLCA
-- S2H
-- OCLCO
-- BD-SySUS
049 ## - LOCAL HOLDINGS (OCLC)
Holding library EQOA
050 #4 - LIBRARY OF CONGRESS CALL NUMBER
Classification number QH442.6
060 #4 - NATIONAL LIBRARY OF MEDICINE CALL NUMBER
Classification number QY 25
072 #7 - SUBJECT CATEGORY CODE
Subject category code SCI
Subject category code subdivision 013060
Source bisacsh
Subject category code TEC
Subject category code subdivision 009010
Source bisacsh
082 04 - DEWEY DECIMAL CLASSIFICATION NUMBER
Classification number 660.65
Edition number 23
Item number TRA
092 ## - LOCALLY ASSIGNED DEWEY CALL NUMBER (OCLC)
Classification number WMS881571700
245 00 - TITLE STATEMENT
Title Transgenic animal technology :
Remainder of title a laboratory handbook /
Statement of responsibility, etc. edited by Carl A. Pinkert.
250 ## - EDITION STATEMENT
Edition statement 3rd ed.
260 ## - PUBLICATION, DISTRIBUTION, ETC. (IMPRINT)
Place of publication, distribution, etc. Burlington :
Name of publisher, distributor, etc. Elsevier Science,
Date of publication, distribution, etc. 2014.
300 ## - PHYSICAL DESCRIPTION
Extent 715 p. ;
Other physical details ill.;
Dimensions 24 cm.
336 ## - CONTENT TYPE
Content type term text
Content type code txt
Source rdacontent
337 ## - MEDIA TYPE
Media type term computer
Media type code c
Source rdamedia
338 ## - CARRIER TYPE
Carrier type term online resource
Carrier type code cr
Source rdacarrier
490 1# - SERIES STATEMENT
Series statement Elsevier insights
500 ## - GENERAL NOTE
General note Section One Overview. Introduction to transgenic animals. Section Two Transgenic animal production focusing on the mouse model. Transgenic animal production using DNA microinjection. Factors affecting transgenic animal production. Gene targeting in embryonic stem cells I: History and methodology. Gene targeting in embryonic stem cells II. Conditional technologies. Retrovirus-mediated gene transfer. Nuclear transfer technologies. Section Three Production of transgenic laboratory and domestic animal species. Production of transgenic rats. Production of transgenic rabbits. Production of transgenic fish. Production of transgenic poultry. Production of transgenic swine by DNA Microinjection. Production of transgenic ruminants by DNA Microinjection. Production of transgenic non-human primates. Retrovirus-mediated gene transfer into oocytes. Section Four: Molecular biology, analyses and enabling technologies Vector design for transgene expression. Analysis of transgene integration. PCR optimization for detection of transgenic integration. Evaluation of transgene expression. Control of gene expression (from standard gain/loss of function to anti-sense to PNAs). Enabling technologies: Cryopreservation of gametes. Enabling technologies: Embryo culture considerations. Databases, Internet Resources and Genetic Nomenclature.
505 0# - FORMATTED CONTENTS NOTE
Formatted contents note Front Cover; Transgenic Animal Technology; Copyright Page; Contents; List of Contributors; Preface; One: Overview; 1 Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; Two: Transgenic Animal Production Focusing on the Mouse Model; 2 DNA Microinjection, Embryo Handling, and Germplasm Preservation; I. Introduction; II. General Methods; A. DNA Preparation and Purification; 1. DNA Construct/Fragment Structure; 2. Factors that Influence Transgenic Mouse Production; 3. Preparation of DNA for Microinjection.
Formatted contents note A. Construction and Isolation of DNAb. DNA Purification; c. Purification and Quantification of DNA; B. Superovulation, Egg Culture, and Harvest; 1. Mouse Strains for Microinjection and Transgenic Models; a. Strains; b. Genetic Variability and Transgene Expression; 2. Superovulation of Mice; a. Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin; b. Follicle Stimulating Hormone and Luteinizing Hormone Delivered with Osmotic Pumps; 3. Production of Eggs from Superovulated Females; a. Colony; b. Influence of Litter Number; 4. In Vitro Culture of Superovulated Eggs; a. Equipment.
Formatted contents note B. Atmosphere for Culturing Eggsc. Glass Pipettes for Manipulating Eggs; d. Media; e. Water Sources; f. Culture Dishes; 5. Harvesting Superovulated Eggs; a. Mating; b. Oviduct Dissection; c. Release of Eggs from the Oviduct; d. Egg Development and Microinjection Timing; e. Efficiency of Egg Culture; C. Microinjection Needles and Slides; 1. Types of Glass; 2. Preparation of Glass; 3. Pipette Pullers; 4. Injection Pipette Shapes; 5. Beveling Hollow Capillary Microinjection Needles; 6. Microforge Shaping of Pipettes; a. Manufacturers; b. Heating Filament; c. Holding Pipette; d. Injection Pipette.
Formatted contents note 7. Microinjection Slide for Eggsa. Slide Designs; b. Microinjection Media; c. Arrangement of DNA and Media Drops; D. Microinjection Equipment; 1. Microscopes; 2. Micromanipulators; a. Micropipette Holders; b. Micromanipulator Adjustments; c. Antivibration Tables; 3. Microinjection Systems; a. General; b. Oil-Driven Injection Assembly; 4. Oil-Driven Holding Pipettes; 5. Electronic Delivery Systems; E. Microinjection Procedure; 1. Preparation of Injection and Holding Pipettes (Micrometer Syringe System); a. Holding Pipette; b. Injection Pipette; c. Aligning the Injection Needle.
Formatted contents note D. Loading DNA into the Injection Needle2. Preparing Eggs for Microinjection; 3. Steps for Efficient Manipulation of Eggs During Microinjection; a. General; b. Micromanipulators; c. Microinjection Needle; d. Controls and Egg Manipulation; 4. Visualizing and Injecting the Egg Pronuclei; a. Orientation; b. Egg Membrane Penetration with the Injection Needle; c. Filling the Pronuclei with DNA Solution; 5. Criteria for Microinjection to Yield Transgenic Mice; a. Treatment After Microinjection; b. Egg Lysis; c. Record Keeping; d. In Vitro Culture of Microinjected Eggs; F. Egg Transfer.
520 ## - SUMMARY, ETC.
Summary, etc. Transgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engine.
588 ## - SOURCE OF DESCRIPTION NOTE
Source of description note Description based on print version record.
650 #0 - SUBJECT ADDED ENTRY--TOPICAL TERM
Topical term or geographic name as entry element Animal genetic engineering.
9 (RLIN) 38568
Topical term or geographic name as entry element Transgenic animals.
9 (RLIN) 38569
Topical term or geographic name as entry element Animal biotechnology.
9 (RLIN) 38570
Topical term or geographic name as entry element Animals, Genetically Modified.
9 (RLIN) 38571
Topical term or geographic name as entry element SCIENCE
General subdivision Chemistry
-- Industrial & Technical.
Source of heading or term bisacsh
9 (RLIN) 38572
Topical term or geographic name as entry element TECHNOLOGY & ENGINEERING
General subdivision Chemical & Biochemical.
Source of heading or term bisacsh
9 (RLIN) 38573
Topical term or geographic name as entry element Animal biotechnology.
Source of heading or term fast
Authority record control number (OCoLC)fst00809103
9 (RLIN) 38570
Topical term or geographic name as entry element Animal genetic engineering.
Source of heading or term fast
Authority record control number (OCoLC)fst00809192
9 (RLIN) 38568
Topical term or geographic name as entry element Transgenic animals.
Source of heading or term fast
Authority record control number (OCoLC)fst01154666
9 (RLIN) 38569
655 #2 - INDEX TERM--GENRE/FORM
Genre/form data or focus term Laboratory Manual.
9 (RLIN) 38574
700 1# - ADDED ENTRY--PERSONAL NAME
Personal name Pinkert, Carl A.,
Relator term editor.
9 (RLIN) 38575
776 08 - ADDITIONAL PHYSICAL FORM ENTRY
Relationship information Erscheint auch als:
Note Druck-Ausgabe
Main entry heading Pinkert, Carl A. Transgenic Animal Technology .
Title A Laboratory Handbook
830 #0 - SERIES ADDED ENTRY--UNIFORM TITLE
Uniform title Elsevier insights.
9 (RLIN) 31665
856 40 - ELECTRONIC LOCATION AND ACCESS
Materials specified ScienceDirect All Books
Uniform Resource Identifier <a href="https://www.sciencedirect.com/science/book/9780124104907">https://www.sciencedirect.com/science/book/9780124104907</a>
942 ## - ADDED ENTRY ELEMENTS (KOHA)
Source of classification or shelving scheme Dewey Decimal Classification
Koha item type Books
Holdings
Withdrawn status Lost status Source of classification or shelving scheme Damaged status Not for loan Home library Current library Shelving location Date acquired Total Checkouts Full call number Barcode Date last seen Copy number Price effective from Koha item type
    Dewey Decimal Classification   Not For Loan Central Library, SUST Central Library, SUST General Stacks 13/06/2022   660.65 TRA 0076334 13/06/2022 1 13/06/2022 Books