TY - BOOK AU - Bjergb�k,Lotte TI - DNA repair protocols T2 - Methods in molecular biology, SN - 9781617799976 (hbk. : alk. paper) U1 - 572.86459 23 PY - 2012/// CY - New York PB - Humana Press/Springer KW - DNA repair KW - Laboratory manuals KW - DNA Repair KW - Laboratory Manuals N1 - Includes bibliographical references and index; DNA repair : front and center and not going away!; Vilhelm A. Bohr --; Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome; Dashnamoorthy Ravi and Alexander James Roy Bishop --; Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening; Lone Vedel Sch�ler, Tine H�rning M�ller, Steffen N�rgaard, Lotte Vestergaard, and Anders Olesn --; Establishment of the DNA repair-defective mutants in DT40 cells; Masamichi Ishiai, Emi Uchida, and Minoru Takata --; Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair; Chun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu --; Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair; G�unter Speit and Andreas Rothfuss --; Detection of DNA damage by comet fluorescence in situ hybridization; Wiebke Schl�ormann and Michael Glei --; Quantitative DNA damage and repair measurement with the yeast comet assay; Rui Oliveira and Bj�orn Johansson --; Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR; Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten --; In vitro DNA mismatch repair in human cells; Liya Gu, Charles M. Ensor, and Guo-Min Li --; Eukaryotic DNA mismatch repair in vitro; Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang --; Immuno-slot blot assay for detection of UVR-mediated DNA damage; Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke --; Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay; Thomas R. Berton and David L. Mitchell --; Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR; Ahmad Besaratinia and Gerd P. Pfeifer --; Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies; Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Sch�arer --; Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts; Puck Knipscheer, Markus R�aschle, Orlando Sch�arer, and Johannes C. Walter --; In vitro base excision repair using mammalian cell extracts; Jason L. Parsons and Grigory L. Dianov --; Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro; Dolores C�ordoba-Ca�nero, Teresa Rold�an-Arjona, and Rafael R. Ariza --; In vitro monitoring of base excision repair in Saccharomyces cerevisiae; Simone Moertl and Fred Ahne --; Mitochondrial base excision repair assays; Ricardo Gredilla and Tinna Stevnsner --; O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks; April M. Luke and Jun Nakamura --; DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis; Helen E. Bryant --; Quantitative amplification of single-stranded DNA; Eva-Maria Holstein and David Lydall --; Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast; James M. Dewar and David Lydall --; Monitoring DNA recombination initiated by HO endonuclease; Neal Sugawara and James E. Haber --; Monitoring I-SceI-induced double-strand break repair in DT40 Cells; David du Plessis and Julian E. Sale --; I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks; Amanda Gunn and Jeremy M. Stark --; Studying repair of a single protein-bound nick in vivo using the Flp-nick system; Ida Nielsen, Anni H. Andersen, and Lotte Bjergb�k --; Measurement of diversification in the immunoglobulin light chain gene of DT40 cells; Julian E. Sale --; Live cell microscopy of DNA damage response in Saccharomyces cerevisiae; Sonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby --; Use of gene targeting to study recombination in mammalian cell DNA repair mutants; Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn --; In vitro rejoining of double strand breaks in genomic DNA; George Iliakis, Emil Mladenov, and Nge Cheong --; An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides; Kamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters --; Assays of bypass replication of genotoxic lesions in cell-free extracts; Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone --; Quantitative measurement of translesion DNA synthesis in mammalian cells; Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh --; Detection of PCNA modifications in Saccharomyces cerevisiae; Adelina A. Davies and Helle D. Ulrich --; In vitro PCNA modification assays; Joanne L. Parker and Helle D. Ulrich --; Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication; George Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang --; CHK1 kinase activity assay; Hong Yan Wang and Ya Wang --; Histone H2AX phosphorylation : a marker for DNA damage; Arishya Sharma, Kamini Singh, and Alexandru Almasan ER -