Handbook of advanced chromatography/mass spectrometry techniques / edited by Michal Holčapek, Wm. Craig Byrdwell.

Includes bibliographical references at the end of each chapters and index.

Front Cover; Handbook of Advanced Chromatography/Mass Spectrometry Techniques; Dedication; Handbook of Advanced Chromatography/Mass Spectrometry TechniquesEdited byMichal HolčapekUniversity of Pardubice, Pardubice ... ; Copyright; Contents; List of Contributors; Preface; 1 -- THEORY AND PRACTICE OF UHPLC AND UHPLC-MS; 1. INTRODUCTION; 2. BRIEF DESCRIPTION OF ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY AND HISTORICAL BACKGROUND; 2.1 INTEREST IN SMALL PARTICLES IN LIQUID CHROMATOGRAPHY; 2.2 INTEREST IN VERY HIGH PRESSURES IN LIQUID CHROMATOGRAPHY.

2.3 PRELIMINARY WORKS OF J.W. JORGENSON AND M.L. LEE IN ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY3. KINETIC COMPARISON OF ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY WITH OTHER EXISTING TECHNOLOGIES FOR FAST AND HIGH-RESOLUTIO ... ; 3.1 BRIEF PRESENTATION OF THE ALTERNATIVE APPROACHES TO ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY; 3.2 BEST LIQUID CHROMATOGRAPHY APPROACH IN ISOCRATIC MODE-THEORY AND APPLICATIONS; 3.3 BEST LIQUID CHROMATOGRAPHY APPROACH IN GRADIENT MODE-THEORY AND APPLICATIONS; 4. PROBLEMS RELATED TO ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY.

4.1 THE NEED TO WORK WITH A DEDICATED INSTRUMENTATION4.2 THE NEED FOR SPECIFIC COLUMNS COMPATIBLE WITH ULTRAHIGH PRESSURES; 4.3 THE CHANGES IN SOLVENT PROPERTIES WITH PRESSURE; 5. METHOD TRANSFER FROM HIGH-PRESSURE LIQUID CHROMATOGRAPHY TO ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY; 5.1 THE RULES FOR ISOCRATIC MODE-THEORY AND APPLICATIONS; 5.2 THE RULES FOR GRADIENT MODE-THEORY AND APPLICATIONS; 6. FIELDS OF APPLICATION FOR ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY AND RELATED ISSUES.

6.1 ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY/MASS SPECTROMETRY FOR HIGH THROUGHPUT IN BIOANALYSIS6.2 HIGH RESOLUTION DRUG METABOLISM BY ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY USING QUADRUPOLE TIME-OF-FLIG ... ; 6.3 ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY FOR MULTIRESIDUE SCREENING; 6.4 ULTRAHIGH-PRESSURE LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY IN METABOLOMICS; 7. CONCLUSION/PERSPECTIVES; REFERENCES; FURTHER READING; 2 -- ADVANCES IN HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY; 1. INTRODUCTION.

2. COLUMNS FOR HYDROPHILIC INTERACTION LIQUID CHROMATOGRAPHY SEPARATIONS2.1 SILICA GEL AND HYBRID INORGANIC SORBENTS; 2.2 CHEMICALLY BONDED SILICA-BASED STATIONARY PHASES; 2.2.1 Amino, Amide Amino Acid, and Peptide Bonded Stationary Phases; 2.2.2 Diol, Polyethylene Glycol, Thioglycerol, Cyclodextrin, and Sugar Bonded Phases; 2.2.3 Polysuccinimide Bonded Stationary Phases; 2.2.4 Other Polymer Coated and Bonded Silica Stationary Phases; 2.2.5 Zwitterionic and Mixed-Mode Silica Stationary Phases; 2.3 AQUEOUS NORMAL-PHASE CHROMATOGRAPHY ON HYDROSILATED SILICA PHASES.

Handbook of Advanced Chromatography /Mass Spectrometry Techniques is a compendium of new and advanced analytical techniques that have been developed in recent years for analysis of all types of molecules in a variety of complex matrices, from foods to fuel to pharmaceuticals and more. Focusing on areas that are becoming widely used or growing rapidly, this is a comprehensive volume that describes both theoretical and practical aspects of advanced methods for analysis. Written by authors who have published the foundational works in the field, the chapters have an emphasis on lipids, but reach a broader audience by including advanced analytical techniques applied to a variety of fields. Handbook of Advanced Chromatography / Mass Spectrometry Techniques is the ideal reference for those just entering the analytical fields covered, but also for those experienced analysts who want a combination of an overview of the techniques plus specific and pragmatic details not often covered in journal reports. The authors provide, in one source, a synthesis of knowledge that is scattered across a multitude of literature articles. The combination of pragmatic hints and tips with theoretical concepts and demonstrated applications provides both breadth and depth to produce a valuable and enduring reference manual. It is well suited for advanced analytical instrumentation students as well as for analysts seeking additional knowledge or a deeper understanding of familiar techniques.

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