Primary and stem cells : gene transfer technologies and applications / edited by Uma Lakshmipathy, Bhaskar Thyagarajan.

Includes bibliographical references and index.

DNA assembly technologies based on homologus recombination / Billyana Tsvetanova [and others] -- Multigene assembly for construction of synthetic operons : creation and delivery of an optimized all-in-one expression construct for generating mouse iPS cells / Takefumi Sone [and others] -- Strategies for the delivery of naked DNA / Jennifer C. Moore and Ronald P. Hart -- Episomal vectors / Junying Yu -- Nonintegrating DNA virus / Chris Kemp and David Kuninger -- Nonintegrating RNA viruses / Noemi Fusaki -- Protein delivery / Xiao-Jian Han and Kazuhito and Tomizawa -- Sleeping beauty transposon-mediated stable gene delivery / Andrew Wilber [and others] -- Integrating viral vectors for gene modifcations / Gemma L. Mendel, Brian Paszkiet, and Rafal P. Witek -- Bacteriophase integrases for site-specific integration / Bhaskar Thyagarajan -- Improving gene targeting efficiency in human pluripotent stem cells / Sangyoon Han [and others] -- Modifed stem cells as disease models and in toxicity screening / Catharina Ellerstrom [and others] -- Screening and drug discovery / Elizabeth A. Davenport and Robert S. Ames.

"This book describes basic cell engineering methods, emphasizing stem cell applications, and use of the genetically modified stem cells in cell therapy and drug discovery. Together, the chapters introduce and offer insights on new techniques for engineering of stem cells and the delivery of transgenes into stem cells via various viral and non-viral systems. The book offers a guide to the types of manipulations currently available to create genetically engineered stem cells that suit any investigator's purpose, whether it's basic science investigation, creation of disease models and screens, or cells for therapeutic applications"--Provided by publisher.