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020 _a9789400745315
_9978-94-007-4531-5
024 7 _a10.1007/978-94-007-4531-5
_2doi
050 4 _aTP248.6
072 7 _aTCBG
_2bicssc
072 7 _aSCI029000
_2bisacsh
082 0 4 _a660.65
_223
245 1 0 _aSite-directed insertion of transgenes
_h[electronic resource] /
_cedited by Sylvaine Renault, Philippe Duchateau.
264 1 _aDordrecht :
_bSpringer Netherlands :
_bImprint: Springer,
_c2013.
300 _aXIV, 394 p.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
490 1 _aTopics in Current Genetics,
_x1610-2096 ;
_v23
505 0 _aPreface -- Section 1: Site–directed integration of transgenes -- 1 Transgene site-specific integration : troubles and solutions -- 2 :  Designing non-viral targetd integrating vectors for genome enginnering in vertebrates -- 3: Gene targeting and homologous recombination in Saccharomyces cerevisiae4: Eucaryotic homologous recombination in mammalian cells -- 5 Engineered Zinc Finger Nucleases for Targeted Genome Editing -- 6: Engineered meganucleases for genome engineering purposes -- Section3: Integration based on Site-specific recombination -- 7 : Cre/loxP, Flp/FRT systems and pluripotent stem cell lines -- 8 : Site-specific recombination using C31 Integrase -- 9 : Modified transposases used for site-directed insertion of transgenes -- 10 : Targeted Plasmid Integration into the Human Genome by Engineered Recombinases -- 11: Gene site-specific insertion in plants -- 12 Site-directed insertion of transgenes in Drosophila -- 13. Nuclease Mediated Targeted Genome Modification in Mammalian Cells -- 14: Bio-applications derived from site-directed genome modification technologies.
520 _aThe post-genomic era has brought new challenges and opportunities in all fields of the biology. In this context, several genome engineering technologies have emerged that will help deciphering genes function by as well as improve gene therapy strategies. Genomic modifications such as knock-in, knock-out, knock-down, sequence replacement or modification can today be routinely performed. However, in front of this large palette of methodologies scientists may experience difficulties to gather useful information’s scattered within the literature. This book aims to present the state of this field from basic mechanisms of site-directed modifications to their applications in a wide range of organisms such as bacteria, yeast, plants, insects, mammals. It will discuss the problems encountered when using the random integration strategy and present the recent advances made in targeted genome modification. Technologies based on Zinc Finger nucleases, Meganucleases, TALEN, CRE and FLP recombinase, C31 integrase, transposases and resolvases are fully detailed with their strengths and weaknesses. All these information’s will help students and experienced researchers to understand and choose the best technology for their own purposes.
650 0 _aChemistry.
650 0 _aGene expression.
650 0 _aGene therapy.
650 0 _aMedical genetics.
650 0 _aGenetic engineering.
650 1 4 _aChemistry.
650 2 4 _aGenetic Engineering.
650 2 4 _aGene Therapy.
650 2 4 _aGene Function.
650 2 4 _aGene Expression.
700 1 _aRenault, Sylvaine.
_eeditor.
700 1 _aDuchateau, Philippe.
_eeditor.
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9789400745308
830 0 _aTopics in Current Genetics,
_x1610-2096 ;
_v23
856 4 0 _uhttp://dx.doi.org/10.1007/978-94-007-4531-5
912 _aZDB-2-SBL
942 _2Dewey Decimal Classification
_ceBooks
999 _c48388
_d48388