000			05894cam a2200673Mi 4500
001			sulb-eb0031371
003			BD-SySUS
005			20170713221256.0
006			m o d
007			cr |n|---|||||
008			120123s2011 nju ob 000 0 eng d
040			_aEBLCP _beng _epn _cEBLCP _dOCLCQ _dUIU _dN$T _dDG1 _dYDXCP _dOCLCQ _dOHI _dOCLCF _dUKDOC _dOCLCQ _dDEBSZ _dOCLCQ _dBD-SySUS
019			_a784138844
020			_a9783527644582 _q(electronic bk.)
020			_a352764458X _q(electronic bk.)
020			_a9783527644773 _q(electronic bk.)
020			_a3527644776 _q(electronic bk.)
029	1		_aAU@ _b000048852576
029	1		_aDEBSZ _b431074321
029	1		_aNZ1 _b15922069
029	1		_aDEBBG _bBV043394030
035			_a(OCoLC)773564828 _z(OCoLC)784138844
037			_a10.1002/9783527644582 _bWiley InterScience _nhttp://www3.interscience.wiley.com
050		4	_aQP624.5.D726 .H889 2011
072		7	_aSCI _x029000 _2bisacsh
082	0	4	_a572.8633
049			_aMAIN
245	0	0	_aTag-based next generation sequencing / _cedited by Matthias Harbers and Günter Kahl.
260			_aHoboken : _bJohn Wiley & Sons, _c2011.
300			_a1 online resource (609 pages)
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
520			_aTag-based approaches were originally designed to increase the throughput of capillary sequencing, where concatemers of short sequences were first used in expression profiling. New Next Generation Sequencing methods largely extended the use of tag-based approaches as the tag lengths perfectly match with the short read length of highly parallel sequencing reactions. Tag-based approaches will maintain their important role in life and biomedical science, because longer read lengths are often not required to obtain meaningful data for many applications. Whereas genome re-sequencing and de novo sequ.
505	0		_aTag-based Next Generation Sequencing; Contents; Preface; List of Contributors; Part One: Tag-Based Nucleic Acid Analysis; 1 DeepSuperSAGE: High-Throughput Transcriptome Sequencing with Now- and Next-Generation Sequencing Technologies; 1.1 Introduction; 1.2 Overview of the Protocols; 1.2.1 Principle of the SuperSAGE Method; 1.2.2 Power of the SuperSAGE Tag; 1.2.3 Development of DeepSuperSAGE; 1.2.4 Ditag-Based DeepSuperSAGE (for 454 Pyrosequencing); 1.2.5 Single-Tag-Based DeepSuperSAGE (HT-SuperSAGE); 1.3 Methods and Protocols; 1.3.1 Linker or Adapter Preparation; 1.3.2 RNA Samples.
505	8		_a1.3.3 cDNA Synthesis and NlaIII Digestion; 1.3.4 Tag Extraction from cDNA; 1.3.5 Tag Extraction from cDNA; 1.3.6 Purification of Linker-Tag Fragments; 1.3.7 Ditag or Adapter-Tag Formation and Amplification; 1.3.8 Preparation of Templates for Sequencing; 1.4 Applications; 1.4.1 Applications of DeepSuperSAGE in Combination with 454 Pyrosequencing; 1.4.2 Practical Analysis of HT-SuperSAGE; 1.5 Perspectives; References; 2 DeepCAGE: Genome-Wide Mapping of Transcription Start Sites; 2.1 Introduction; 2.2 What is CAGE?; 2.3 Why CAGE?; 2.4 Methods and Protocols; 2.4.1 Key Reagents and Consumables.
505	8		_a2.4.2 Precautions; 2.4.3 RNA Samples Used for DeepCAGE Library Preparation; 2.4.4 DeepCAGE Library Preparation; 2.5 Applications; 2.6 Perspectives; References; 3 Definition of Promotome-Transcriptome Architecture Using CAGEscan; 3.1 Introduction; 3.2 What is CAGEscan?; 3.3 Why CAGEscan?; 3.4 Methods and Protocols; 3.4.1 Key Reagents and Consumables; 3.4.2 Precautions; 3.4.3 RNA Samples Used for CAGEscan Library Preparation; 3.4.4 Considerations on Pooling CAGEscan Libraries; 3.4.5 CAGEscan Library Preparation; 3.5 Applications and Perspectives; References.
505	8		_a4 RACE: New Applications of an Old Method to Connect Exons; 4.1 Introduction; 4.2 Deep-RACE; 4.2.1 Choice of the Sequencer; 4.2.2 Validation of Promoter Studies; 4.2.3 Other Applications of Deep-RACE; 4.2.4 Limitations of Deep-RACE; 4.3 Methods Outline; 4.3.1 Primer Design; 4.3.2 Molecular Biology of Deep-RACE Library Preparation; 4.3.3 Sequencing of Deep-RACE Libraries; 4.3.4 Analysis; 4.4 Perspectives; References; 5 RNA-PET: Full-Length Transcript Analysis Using 5'- and 3'-Paired-End Tag Next-Generation Sequencing; 5.1 Introduction; 5.2 Methods and Protocols.
505	8		_a5.2.1 Key Reagents and Consumables; 5.2.2 Protocol; 5.3 Applications; 5.3.1 PET Sequencing with SOLiD; 5.3.2 Mapping of the PETs; 5.3.3 PET Clustering, Annotation, and Genome Browser Visualization; 5.4 Perspectives; References; 6 Stranded RNA-Seq: Strand-Specific Shotgun Sequencing of RNA; 6.1 Introduction; 6.1.1 Before Starting; 6.2 Methods and Protocols; 6.2.1 Preface; 6.2.2 Materials and Consumables; 6.2.3 Protocol; 6.3 Bioinformatic Considerations; 6.4 Applications; 6.5 Perspectives; References.
505	8		_a7 Differential RNA Sequencing (dRNA-Seq): Deep-Sequencing-Based Analysis of Primary Transcriptomes.
588	0		_aPrint version record.
650		0	_aDNA microarrays.
650		0	_aNeurobiology.
650		4	_aOligonucleotide array sequence analysis.
650		7	_aSCIENCE _xLife Sciences _xGenetics & Genomics. _2bisacsh
650		7	_aDNA microarrays. _2fast _0(OCoLC)fst00886592
650		7	_aNeurobiology. _2fast _0(OCoLC)fst01036315
655		4	_aElectronic books.
700	1		_aHarbers, Matthias.
700	1		_aKahl, Günter.
776	0	8	_iPrint version: _tTag-based Next Generation Sequencing. _dHoboken : John Wiley & Sons, ©2011 _z9783527328192
856	4	0	_uhttp://onlinelibrary.wiley.com/book/10.1002/9783527644582 _zWiley Online Library [Free Download only for SUST IP]
938			_a123Library _b123L _n31772
938			_aEBL - Ebook Library _bEBLB _nEBL822736
938			_aEBSCOhost _bEBSC _n413420
938			_aYBP Library Services _bYANK _n7484191
938			_aYBP Library Services _bYANK _n7362809
994			_a92 _bDG1
999			_c63023 _d63023