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020 _a9783527687978
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020 _a3527687971
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020 _a9783527687992
_q(electronic bk.)
020 _a3527687998
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020 _a3527335161
020 _a9783527335169
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029 1 _aAU@
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035 _a(OCoLC)904046277
_z(OCoLC)961696441
_z(OCoLC)962730044
050 4 _aTD427.M53
072 7 _aTEC
_x010000
_2bisacsh
082 0 4 _a628.1662
_223
049 _aMAIN
245 0 0 _aModern techniques for pathogen detection /
_cedited by Jürgen Popp and Michael Bauer.
264 1 _aWeinheim, Germany :
_bWiley Blackwell,
_c2015.
300 _a1 online resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
588 0 _aVendor-supplied metadata.
500 _aIncludes index.
504 _aIncludes bibliographical references at the end of each chapters and index.
505 0 _aRelated Titles; Title Page; Copyright; Table of Contents; Preface; List of Contributors; Chapter 1: Unmet Medical Needs in Life-Threatening Infections -- Caring for the Critically Ill; 1.1 Life Threatening Infections and Sepsis -- Defining the Problem; 1.2 Sepsis as a "Hidden Healthcare Disaster"; 1.3 Microorganisms and Types of Infection Triggering Sepsis; 1.4 Emerging Problems Related to Resistance in Bacterial Infections; 1.5 The Role of Fungi and Viruses; 1.6 The Need for New Approaches in Diagnostics of Life-Threatening Infection and Sepsis
505 8 _a1.7 Rapid and Sensitive Culture-Independent Strategies to Identify Blood Stream Infection1.8 Beyond Infection -- Profiling the Immune Response of the Septic Host; 1.9 Host Factors Contributing to Pathogenesis of Sepsis; References; Chapter 2: Identification Methods -- An Overview; 2.1 Taxonomy of Pathogenic Organisms; 2.2 Microscopic Methods; 2.3 Culture-Based Methods; 2.4 Nucleic Acid-Based Techniques; 2.5 Serology; 2.6 Conclusions and Perspectives; References; Chapter 3: Nucleic Acid Amplification Techniques; 3.1 Introduction; 3.2 The Basic PCR Protocol; 3.3 Primer Design
505 8 _a3.4 Modified End-Point PCR Protocols3.5 Non-PCR NAT: Isothermal Amplification Protocols; 3.6 Quantitative PCR; 3.7 Controls, Probing, and General Aspects of Result Interpretation; 3.8 Preanalytics; 3.9 Fields of PCR Application; 3.10 Microbial Trace Detection in BSI, Ascites, and Synovial Fluids; 3.11 Upcoming Routine Solutions -- Dawn of Assay Automation; 3.12 Conclusion and Perspective; Acknowledgment; References; Chapter 4: DNA Microarrays for Pathogen Detection; 4.1 Introduction; 4.2 DNA Microarrays for the Detection of Bacterial Pathogens; 4.3 Antibiotic Resistance Detection
505 8 _a4.4 DNA Microarrays for Virus Diagnostics4.5 DNA Microarrays for Detection of Fungal Pathogens; 4.6 DNA Microarrays for Parasite Diagnostics; 4.7 Conclusion an Outlook; References; Chapter 5: MALDI-ToF; 5.1 Introduction; 5.2 MALDI-ToF Technology; 5.3 Bacterial Identification Using Mass Spectrometry; 5.4 Culture-Independent Rapid Identification of Clinical Pathogens; 5.5 Antibiotic Susceptibility Testing Using Mass Spectrometry; References; Chapter 6: IR and Raman Spectroscopy for Pathogen Detection; 6.1 Introduction; 6.2 Bulk Analysis; 6.3 Excitation with Visible Wavelengths
505 8 _a6.4 UV-Resonance Raman Spectroscopy6.5 Single-Cell Analyses; 6.6 Conclusion and Outlook; References; Chapter 7: FISH; 7.1 Introduction; 7.2 FISH Techniques; 7.3 Clinical Implications of PNA FISH; 7.4 Summary and Outlook; References; Chapter 8: Conclusions; Index; End User License Agreement
650 0 _aPathogenic microorganisms
_xDetection.
650 7 _aTECHNOLOGY & ENGINEERING
_xEnvironmental
_xGeneral.
_2bisacsh
650 7 _aPathogenic microorganisms
_xDetection.
_2fast
_0(OCoLC)fst01054936
650 0 4 _aMicroorganismos patógenos
_xIdentificación.
655 4 _aElectronic books.
655 0 _aElectronic books.
700 1 _aPopp, Jürgen,
_eeditor.
700 1 _aBauer, Michael,
_eeditor.
776 0 8 _iPrint version:
_z9783527687978
_w(OCoLC)904046277
856 4 0 _uhttp://onlinelibrary.wiley.com/book/10.1002/9783527687978
_zWiley Online Library [Free Download only for SUST IP]
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