000 06373nam a22003017a 4500
001 sulb0072903
003 BD-SySUS
005 20191209084926.0
008 191209s2012 nyua b 001 0 eng d
020 _a9781617799976 (hbk. : alk. paper)
020 _a1617799971 (hbk. : alk. paper)
040 _aUKMGB
_beng
_cUKMGB
_dRML
_dCOU
_dBTCTA
_dYDXCP
_dBWX
_dCOO
_dMUU
_dDLC
_dBD-SySUS
082 0 4 _a572.86459
_223
_bDNA
245 0 0 _aDNA repair protocols /
_cedited by Lotte Bjergb�k.
250 _a3rd ed.
260 _aNew York :
_bHumana Press/Springer,
_c©2012.
300 _axvii, 631 p. :
_bill. (some col.) ;
_c27 cm.
490 1 _aMethods in molecular biology,
_x1064-3745 ;
_v920
490 1 _aSpringer protocols
504 _aIncludes bibliographical references and index.
505 0 0 _tDNA repair : front and center and not going away! /
_rVilhelm A. Bohr --
_tIdentification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome /
_rDashnamoorthy Ravi and Alexander James Roy Bishop --
_tIsolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening /
_rLone Vedel Sch�ler, Tine H�rning M�ller, Steffen N�rgaard, Lotte Vestergaard, and Anders Olesn --
_tEstablishment of the DNA repair-defective mutants in DT40 cells /
_rMasamichi Ishiai, Emi Uchida, and Minoru Takata --
_tElectrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair /
_rChun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu --
_tComet assay : a sensitive genotoxicity test for the detection of DNA damage and repair /
_rG�unter Speit and Andreas Rothfuss --
_tDetection of DNA damage by comet fluorescence in situ hybridization /
_rWiebke Schl�ormann and Michael Glei --
_tQuantitative DNA damage and repair measurement with the yeast comet assay /
_rRui Oliveira and Bj�orn Johansson --
_tAnalysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR /
_rAmy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten --
_tIn vitro DNA mismatch repair in human cells /
_rLiya Gu, Charles M. Ensor, and Guo-Min Li --
_tEukaryotic DNA mismatch repair in vitro /
_rFenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang --
_tImmuno-slot blot assay for detection of UVR-mediated DNA damage /
_rMahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke --
_tQuantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay /
_rThomas R. Berton and David L. Mitchell --
_tMeasuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR /
_rAhmad Besaratinia and Gerd P. Pfeifer --
_tConstruction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies /
_rMilica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Sch�arer --
_tReplication-coupled DNA interstrand cross-link repair in Xenopus egg extracts /
_rPuck Knipscheer, Markus R�aschle, Orlando Sch�arer, and Johannes C. Walter --
_tIn vitro base excision repair using mammalian cell extracts /
_rJason L. Parsons and Grigory L. Dianov --
_tUsing Arabidopsis cell extracts to monitor repair of DNA base damage in vitro /
_rDolores C�ordoba-Ca�nero, Teresa Rold�an-Arjona, and Rafael R. Ariza --
_tIn vitro monitoring of base excision repair in Saccharomyces cerevisiae /
_rSimone Moertl and Fred Ahne --
_tMitochondrial base excision repair assays /
_rRicardo Gredilla and Tinna Stevnsner --
_tO-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks /
_rApril M. Luke and Jun Nakamura --
_tDNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis /
_rHelen E. Bryant --
_tQuantitative amplification of single-stranded DNA /
_rEva-Maria Holstein and David Lydall --
_tSimple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast /
_rJames M. Dewar and David Lydall --
_tMonitoring DNA recombination initiated by HO endonuclease /
_rNeal Sugawara and James E. Haber --
_tMonitoring I-SceI-induced double-strand break repair in DT40 Cells /
_rDavid du Plessis and Julian E. Sale --
_tI-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks /
_rAmanda Gunn and Jeremy M. Stark --
_tStudying repair of a single protein-bound nick in vivo using the Flp-nick system /
_rIda Nielsen, Anni H. Andersen, and Lotte Bjergb�k --
_tMeasurement of diversification in the immunoglobulin light chain gene of DT40 cells /
_rJulian E. Sale --
_tLive cell microscopy of DNA damage response in Saccharomyces cerevisiae /
_rSonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby --
_tUse of gene targeting to study recombination in mammalian cell DNA repair mutants /
_rJennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn --
_tIn vitro rejoining of double strand breaks in genomic DNA /
_rGeorge Iliakis, Emil Mladenov, and Nge Cheong --
_tAn in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides /
_rKamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters --
_tAssays of bypass replication of genotoxic lesions in cell-free extracts /
_rNana Nikolaishvili-Feinberg and Marila Cordeiro-Stone --
_tQuantitative measurement of translesion DNA synthesis in mammalian cells /
_rOmer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh --
_tDetection of PCNA modifications in Saccharomyces cerevisiae /
_rAdelina A. Davies and Helle D. Ulrich --
_tIn vitro PCNA modification assays /
_rJoanne L. Parker and Helle D. Ulrich --
_tAnalysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication /
_rGeorge Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang --
_tCHK1 kinase activity assay /
_rHong Yan Wang and Ya Wang --
_tHistone H2AX phosphorylation : a marker for DNA damage /
_rArishya Sharma, Kamini Singh, and Alexandru Almasan.
650 0 _aDNA repair
_vLaboratory manuals.
_933978
650 1 2 _aDNA Repair
_vLaboratory Manuals.
_933979
700 1 _aBjergb�k, Lotte.
_933980
830 0 _aMethods in molecular biology (Clifton, N.J.) ;
_v920.
_933981
830 0 _aSpringer protocols.
_933982
942 _2ddc
_cBK
999 _c74771
_d74771