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008			140623s2014 onc o 000 0 eng d
020			_a9780124095366 _q(electronic bk.)
020			_a0124095364 _q(electronic bk.)
020			_a9780124104907 _q(electronic bk.)
020			_a0124104908 _q(electronic bk.)
020			_z9780124095366
020			_z0124095364
035			_a(OCoLC)881571700 _z(OCoLC)961202138 _z(OCoLC)1076563831 _z(OCoLC)1105197705 _z(OCoLC)1105574825
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082	0	4	_a660.65 _223 _bTRA
092			_aWMS881571700
245	0	0	_aTransgenic animal technology : _ba laboratory handbook / _cedited by Carl A. Pinkert.
250			_a3rd ed.
260			_aBurlington : _bElsevier Science, _c2014.
300			_a715 p. ; _bill.; _c24 cm.
336			_atext _btxt _2rdacontent
337			_acomputer _bc _2rdamedia
338			_aonline resource _bcr _2rdacarrier
490	1		_aElsevier insights
500			_aSection One Overview. Introduction to transgenic animals. Section Two Transgenic animal production focusing on the mouse model. Transgenic animal production using DNA microinjection. Factors affecting transgenic animal production. Gene targeting in embryonic stem cells I: History and methodology. Gene targeting in embryonic stem cells II. Conditional technologies. Retrovirus-mediated gene transfer. Nuclear transfer technologies. Section Three Production of transgenic laboratory and domestic animal species. Production of transgenic rats. Production of transgenic rabbits. Production of transgenic fish. Production of transgenic poultry. Production of transgenic swine by DNA Microinjection. Production of transgenic ruminants by DNA Microinjection. Production of transgenic non-human primates. Retrovirus-mediated gene transfer into oocytes. Section Four: Molecular biology, analyses and enabling technologies Vector design for transgene expression. Analysis of transgene integration. PCR optimization for detection of transgenic integration. Evaluation of transgene expression. Control of gene expression (from standard gain/loss of function to anti-sense to PNAs). Enabling technologies: Cryopreservation of gametes. Enabling technologies: Embryo culture considerations. Databases, Internet Resources and Genetic Nomenclature.
505	0		_aFront Cover; Transgenic Animal Technology; Copyright Page; Contents; List of Contributors; Preface; One: Overview; 1 Introduction to Transgenic Animal Technology; I. Introduction; II. Historical Background; III. Applications and Overview of Text; References; Two: Transgenic Animal Production Focusing on the Mouse Model; 2 DNA Microinjection, Embryo Handling, and Germplasm Preservation; I. Introduction; II. General Methods; A. DNA Preparation and Purification; 1. DNA Construct/Fragment Structure; 2. Factors that Influence Transgenic Mouse Production; 3. Preparation of DNA for Microinjection.
505	8		_aA. Construction and Isolation of DNAb. DNA Purification; c. Purification and Quantification of DNA; B. Superovulation, Egg Culture, and Harvest; 1. Mouse Strains for Microinjection and Transgenic Models; a. Strains; b. Genetic Variability and Transgene Expression; 2. Superovulation of Mice; a. Pregnant Mare Serum Gonadotropin and Human Chorionic Gonadotropin; b. Follicle Stimulating Hormone and Luteinizing Hormone Delivered with Osmotic Pumps; 3. Production of Eggs from Superovulated Females; a. Colony; b. Influence of Litter Number; 4. In Vitro Culture of Superovulated Eggs; a. Equipment.
505	8		_aB. Atmosphere for Culturing Eggsc. Glass Pipettes for Manipulating Eggs; d. Media; e. Water Sources; f. Culture Dishes; 5. Harvesting Superovulated Eggs; a. Mating; b. Oviduct Dissection; c. Release of Eggs from the Oviduct; d. Egg Development and Microinjection Timing; e. Efficiency of Egg Culture; C. Microinjection Needles and Slides; 1. Types of Glass; 2. Preparation of Glass; 3. Pipette Pullers; 4. Injection Pipette Shapes; 5. Beveling Hollow Capillary Microinjection Needles; 6. Microforge Shaping of Pipettes; a. Manufacturers; b. Heating Filament; c. Holding Pipette; d. Injection Pipette.
505	8		_a7. Microinjection Slide for Eggsa. Slide Designs; b. Microinjection Media; c. Arrangement of DNA and Media Drops; D. Microinjection Equipment; 1. Microscopes; 2. Micromanipulators; a. Micropipette Holders; b. Micromanipulator Adjustments; c. Antivibration Tables; 3. Microinjection Systems; a. General; b. Oil-Driven Injection Assembly; 4. Oil-Driven Holding Pipettes; 5. Electronic Delivery Systems; E. Microinjection Procedure; 1. Preparation of Injection and Holding Pipettes (Micrometer Syringe System); a. Holding Pipette; b. Injection Pipette; c. Aligning the Injection Needle.
505	8		_aD. Loading DNA into the Injection Needle2. Preparing Eggs for Microinjection; 3. Steps for Efficient Manipulation of Eggs During Microinjection; a. General; b. Micromanipulators; c. Microinjection Needle; d. Controls and Egg Manipulation; 4. Visualizing and Injecting the Egg Pronuclei; a. Orientation; b. Egg Membrane Penetration with the Injection Needle; c. Filling the Pronuclei with DNA Solution; 5. Criteria for Microinjection to Yield Transgenic Mice; a. Treatment After Microinjection; b. Egg Lysis; c. Record Keeping; d. In Vitro Culture of Microinjected Eggs; F. Egg Transfer.
520			_aTransgenic animal technologies and the ability to introduce functional genes into animals have revolutionized our ability to address complex biomedical and biological questions. This well-illustrated handbook covers the technical aspects of gene transfer - from molecular methods to whole animal considerations - for important laboratory and domestic animal species. It describes methodologies as employed by leading laboratories and is a key resource for researchers, as well as a tool for training technicians and students. This second edition incorporates updates on a variety of genetic engine.
588			_aDescription based on print version record.
650		0	_aAnimal genetic engineering. _938568
650		0	_aTransgenic animals. _938569
650		0	_aAnimal biotechnology. _938570
650		2	_aAnimals, Genetically Modified. _938571
650		7	_aSCIENCE _xChemistry _xIndustrial & Technical. _2bisacsh _938572
650		7	_aTECHNOLOGY & ENGINEERING _xChemical & Biochemical. _2bisacsh _938573
650		7	_aAnimal biotechnology. _2fast _0(OCoLC)fst00809103 _938570
650		7	_aAnimal genetic engineering. _2fast _0(OCoLC)fst00809192 _938568
650		7	_aTransgenic animals. _2fast _0(OCoLC)fst01154666 _938569
655		2	_aLaboratory Manual. _938574
700	1		_aPinkert, Carl A., _eeditor. _938575
776	0	8	_iErscheint auch als: _nDruck-Ausgabe _aPinkert, Carl A. Transgenic Animal Technology . _tA Laboratory Handbook
830		0	_aElsevier insights. _931665
856	4	0	_3ScienceDirect All Books _uhttps://www.sciencedirect.com/science/book/9780124104907
942			_2ddc _cBK
999			_c76300 _d76300