DNA repair protocols / edited by Lotte Bjergb�k.

Includes bibliographical references and index.

DNA repair : front and center and not going away! / Vilhelm A. Bohr -- Identification of genes required for damage survival using a cell-based RNAi screen against the Drosophila genome / Dashnamoorthy Ravi and Alexander James Roy Bishop -- Isolating genes involved with genotoxic drug response in the nematode Caenorhabditis elegans using genome-wide RNAi screening / Lone Vedel Sch�ler, Tine H�rning M�ller, Steffen N�rgaard, Lotte Vestergaard, and Anders Olesn -- Establishment of the DNA repair-defective mutants in DT40 cells / Masamichi Ishiai, Emi Uchida, and Minoru Takata -- Electrophoretic mobility shift assays for protein-DNA complexes involved in DNA repair / Chun Tsai, Vaughn Smider, Byung Joon Hwang, and Gilbert Chu -- Comet assay : a sensitive genotoxicity test for the detection of DNA damage and repair / G�unter Speit and Andreas Rothfuss -- Detection of DNA damage by comet fluorescence in situ hybridization / Wiebke Schl�ormann and Michael Glei -- Quantitative DNA damage and repair measurement with the yeast comet assay / Rui Oliveira and Bj�orn Johansson -- Analysis of DNA damage and repair in nuclear and mitochondrial DNA of animal cells using quantitative PCR / Amy M. Furda, Amanda Smith Bess, Joel N. Meyer, and Bennett Van Houten -- In vitro DNA mismatch repair in human cells / Liya Gu, Charles M. Ensor, and Guo-Min Li -- Eukaryotic DNA mismatch repair in vitro / Fenghua Yuan, Limin Song, Fengsong Liu, Liya Gu, and Yanbin Zhang -- Immuno-slot blot assay for detection of UVR-mediated DNA damage / Mahsa Karbaschi, Nigel J. Brady, Mark D. Evans, and Marcus S. Cooke -- Quantification of DNA photoproducts in mammalian cell DNA using radioimmunoassay / Thomas R. Berton and David L. Mitchell -- Measuring the formation and repair of UV damage at the DNA sequence level by ligation-mediated PCR / Ahmad Besaratinia and Gerd P. Pfeifer -- Construction of plasmids containing site-specific DNA interstrand cross-links for biochemical and cell biological studies / Milica Enoiu, The Vinh Ho, David T. Long, Johannes C. Walter, and Orlando D. Sch�arer -- Replication-coupled DNA interstrand cross-link repair in Xenopus egg extracts / Puck Knipscheer, Markus R�aschle, Orlando Sch�arer, and Johannes C. Walter -- In vitro base excision repair using mammalian cell extracts / Jason L. Parsons and Grigory L. Dianov -- Using Arabidopsis cell extracts to monitor repair of DNA base damage in vitro / Dolores C�ordoba-Ca�nero, Teresa Rold�an-Arjona, and Rafael R. Ariza -- In vitro monitoring of base excision repair in Saccharomyces cerevisiae / Simone Moertl and Fred Ahne -- Mitochondrial base excision repair assays / Ricardo Gredilla and Tinna Stevnsner -- O-hydroxylamine-coupled alkaline gel electrophoresis assay for the detection and measurement of DNA single-strand breaks / April M. Luke and Jun Nakamura -- DNA double-strand break damage and repair assessed by pulsed-field gel electrophoresis / Helen E. Bryant -- Quantitative amplification of single-stranded DNA / Eva-Maria Holstein and David Lydall -- Simple, non-radioactive measurement of single-stranded DNA at telomeric, sub-telomeric, and genomic loci in budding yeast / James M. Dewar and David Lydall -- Monitoring DNA recombination initiated by HO endonuclease / Neal Sugawara and James E. Haber -- Monitoring I-SceI-induced double-strand break repair in DT40 Cells / David du Plessis and Julian E. Sale -- I-SceI-based assays to examine distinct repair outcomes of mammalian chromosomal double strand breaks / Amanda Gunn and Jeremy M. Stark -- Studying repair of a single protein-bound nick in vivo using the Flp-nick system / Ida Nielsen, Anni H. Andersen, and Lotte Bjergb�k -- Measurement of diversification in the immunoglobulin light chain gene of DT40 cells / Julian E. Sale -- Live cell microscopy of DNA damage response in Saccharomyces cerevisiae / Sonia Silva, Irene Gallina, Nadine Eckert-Boulet, and Michael Lisby -- Use of gene targeting to study recombination in mammalian cell DNA repair mutants / Jennifer J. Rahn, Gerald M. Adair, and Rodney S. Nairn -- In vitro rejoining of double strand breaks in genomic DNA / George Iliakis, Emil Mladenov, and Nge Cheong -- An in vitro DNA double-strand break repair assay based on end-joining of defined duplex oligonucleotides / Kamal Datta, Shubhadeep Purkayastha, Ronald D. Neumann, and Thomas A. Winters -- Assays of bypass replication of genotoxic lesions in cell-free extracts / Nana Nikolaishvili-Feinberg and Marila Cordeiro-Stone -- Quantitative measurement of translesion DNA synthesis in mammalian cells / Omer Ziv, Noam Diamant, Sigal Shachar, Ayal Hendel, and Zvi Livneh -- Detection of PCNA modifications in Saccharomyces cerevisiae / Adelina A. Davies and Helle D. Ulrich -- In vitro PCNA modification assays / Joanne L. Parker and Helle D. Ulrich -- Analysis of inhibition of DNA replication in irradiated cells using the SV40 based in vitro assay of DNA replication / George Iliakis, Emil Mladenov, Ya Wang, and Hong Yan Wang -- CHK1 kinase activity assay / Hong Yan Wang and Ya Wang -- Histone H2AX phosphorylation : a marker for DNA damage / Arishya Sharma, Kamini Singh, and Alexandru Almasan.